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pspax2 packaging vector addgene 12260 pmd2 g packaging vector addgene 12259 oligonucleotides dcas13d carmn sgrna  (Addgene inc)


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    Structured Review

    Addgene inc pspax2 packaging vector addgene 12260 pmd2 g packaging vector addgene 12259 oligonucleotides dcas13d carmn sgrna
    Pspax2 Packaging Vector Addgene 12260 Pmd2 G Packaging Vector Addgene 12259 Oligonucleotides Dcas13d Carmn Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15695 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+vector/psPAX2+(Plasmid+%2312260)/pm42850414-194-819-822
    Average 98 stars, based on 15695 article reviews
    pspax2 packaging vector addgene 12260 pmd2 g packaging vector addgene 12259 oligonucleotides dcas13d carmn sgrna - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Truncated CD19 as a selection marker for the isolation of stem cell derived β-cells.
    Article Snippet: .. For the donor vector we cloned 800bp homology arms into the KpnI/NheI and AscI/NotI sites of Addgene vector #31938 (Hockemeyer et al., 2011). ..

    Article Title: Enhancing proteasome activity by NMDAR antagonists explains their therapeutic effect in neurodegenerative and mental diseases.
    Article Snippet: .. Preparation of Hep3B cells stably expressing Ub-R-YFP The Ub-R-YFP open reading frame was excised from an Addgene vector (#11,948) using NheI and NotI, bluntended, and cloned into pBSK-clo2D pre-digested with EcoRV. ..

    Article Title: RBP47 family members are negative regulators of heat stress tolerance in Arabidopsis thaliana
    Article Snippet: RBP47b (AT3G19130) DNA sequence without stop codon, flanked by AttL1 and AttL2 recombinant sites, was synthesized (GeneScript Biotech, Singapore). .. The UBQ10 promoter sequence was PCR amplified using as a template the Addgene vector #127369, using PhusionTM High-Fidelity DNA Polymerase (Thermo, F-530XL), and the resultant amplicon was subsequently cloned into pENTRTM 5’-TOPOTM vector (Thermo, K59120). ..

    Article Title: Enhancing proteasome activity by NMDAR antagonists explains their therapeutic effect in neurodegenerative and mental diseases
    Article Snippet: .. The Ub-R-YFP open reading frame was excised from an Addgene vector (#11,948) using NheI and NotI, blunt-ended, and cloned into pBSK-clo2D pre-digested with EcoRV. ..

    Article Title: Development of super-infective ternary vector systems for enhancing the Agrobacterium -mediated plant transformation and genome editing efficiency
    Article Snippet: The gusA gene encodes β-glucuronidase for the GUS construct, which was amplified from Addgene vector 50 332. .. The Clover GFP gene was amplified from Addgene vector 40 259 for the GFP construct. ..

    Clone Assay:

    Article Title: Truncated CD19 as a selection marker for the isolation of stem cell derived β-cells.
    Article Snippet: .. For the donor vector we cloned 800bp homology arms into the KpnI/NheI and AscI/NotI sites of Addgene vector #31938 (Hockemeyer et al., 2011). ..

    Article Title: Boosting Proteasome Activity: A Novel Mechanism of NMDAR Blockers Against Neurodegeneration
    Article Snippet: The Ub-R-YFP open reading frame was excised from an Addgene vector (#11948) using NheI and NotI, blunt-ended, and cloned into pBSK-clo2D pre-digested with EcoRV. .. The Ub-R-YFP open reading frame was excised from an Addgene vector (#11948) using NheI and NotI, blunt-ended, and cloned into pBSK-clo2D pre-digested with EcoRV. ..

    Article Title: Enhancing proteasome activity by NMDAR antagonists explains their therapeutic effect in neurodegenerative and mental diseases.
    Article Snippet: .. Preparation of Hep3B cells stably expressing Ub-R-YFP The Ub-R-YFP open reading frame was excised from an Addgene vector (#11,948) using NheI and NotI, bluntended, and cloned into pBSK-clo2D pre-digested with EcoRV. ..

    Article Title: RBP47 family members are negative regulators of heat stress tolerance in Arabidopsis thaliana
    Article Snippet: RBP47b (AT3G19130) DNA sequence without stop codon, flanked by AttL1 and AttL2 recombinant sites, was synthesized (GeneScript Biotech, Singapore). .. The UBQ10 promoter sequence was PCR amplified using as a template the Addgene vector #127369, using PhusionTM High-Fidelity DNA Polymerase (Thermo, F-530XL), and the resultant amplicon was subsequently cloned into pENTRTM 5’-TOPOTM vector (Thermo, K59120). ..

    Article Title: Enhancing proteasome activity by NMDAR antagonists explains their therapeutic effect in neurodegenerative and mental diseases
    Article Snippet: .. The Ub-R-YFP open reading frame was excised from an Addgene vector (#11,948) using NheI and NotI, blunt-ended, and cloned into pBSK-clo2D pre-digested with EcoRV. ..

    Stable Transfection:

    Article Title: Enhancing proteasome activity by NMDAR antagonists explains their therapeutic effect in neurodegenerative and mental diseases.
    Article Snippet: .. Preparation of Hep3B cells stably expressing Ub-R-YFP The Ub-R-YFP open reading frame was excised from an Addgene vector (#11,948) using NheI and NotI, bluntended, and cloned into pBSK-clo2D pre-digested with EcoRV. ..

    Expressing:

    Article Title: Enhancing proteasome activity by NMDAR antagonists explains their therapeutic effect in neurodegenerative and mental diseases.
    Article Snippet: .. Preparation of Hep3B cells stably expressing Ub-R-YFP The Ub-R-YFP open reading frame was excised from an Addgene vector (#11,948) using NheI and NotI, bluntended, and cloned into pBSK-clo2D pre-digested with EcoRV. ..

    Sequencing:

    Article Title: RBP47 family members are negative regulators of heat stress tolerance in Arabidopsis thaliana
    Article Snippet: RBP47b (AT3G19130) DNA sequence without stop codon, flanked by AttL1 and AttL2 recombinant sites, was synthesized (GeneScript Biotech, Singapore). .. The UBQ10 promoter sequence was PCR amplified using as a template the Addgene vector #127369, using PhusionTM High-Fidelity DNA Polymerase (Thermo, F-530XL), and the resultant amplicon was subsequently cloned into pENTRTM 5’-TOPOTM vector (Thermo, K59120). ..

    Polymerase Chain Reaction:

    Article Title: RBP47 family members are negative regulators of heat stress tolerance in Arabidopsis thaliana
    Article Snippet: RBP47b (AT3G19130) DNA sequence without stop codon, flanked by AttL1 and AttL2 recombinant sites, was synthesized (GeneScript Biotech, Singapore). .. The UBQ10 promoter sequence was PCR amplified using as a template the Addgene vector #127369, using PhusionTM High-Fidelity DNA Polymerase (Thermo, F-530XL), and the resultant amplicon was subsequently cloned into pENTRTM 5’-TOPOTM vector (Thermo, K59120). ..

    Amplification:

    Article Title: RBP47 family members are negative regulators of heat stress tolerance in Arabidopsis thaliana
    Article Snippet: RBP47b (AT3G19130) DNA sequence without stop codon, flanked by AttL1 and AttL2 recombinant sites, was synthesized (GeneScript Biotech, Singapore). .. The UBQ10 promoter sequence was PCR amplified using as a template the Addgene vector #127369, using PhusionTM High-Fidelity DNA Polymerase (Thermo, F-530XL), and the resultant amplicon was subsequently cloned into pENTRTM 5’-TOPOTM vector (Thermo, K59120). ..

    Article Title: Development of super-infective ternary vector systems for enhancing the Agrobacterium -mediated plant transformation and genome editing efficiency
    Article Snippet: The gusA gene encodes β-glucuronidase for the GUS construct, which was amplified from Addgene vector 50 332. .. The Clover GFP gene was amplified from Addgene vector 40 259 for the GFP construct. ..

    Derivative Assay:

    Article Title: Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects.
    Article Snippet: .. Briefly, the reporter cell linewas created by replacing the mApple fluorophore in the Apple-53BP1trunc Addgene vector #69531, a gift from Ralph Weissleder74, with a mMaroon1 fluorophore derived from the Addgene vector #83842, a gift from Michael Lin79. ..

    Article Title: Mitochondria-derived nuclear ATP surge protects against confinement-induced proliferation defects
    Article Snippet: .. Briefly, the reporter cell line was created by replacing the mApple fluorophore in the Apple-53BP1trunc Addgene vector #69531, a gift from Ralph Weissleder , with a mMaroon1 fluorophore derived from the Addgene vector #83842, a gift from Michael Lin . ..

    Construct:

    Article Title: Development of super-infective ternary vector systems for enhancing the Agrobacterium -mediated plant transformation and genome editing efficiency
    Article Snippet: The gusA gene encodes β-glucuronidase for the GUS construct, which was amplified from Addgene vector 50 332. .. The Clover GFP gene was amplified from Addgene vector 40 259 for the GFP construct. ..



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